Protein Purification
Baculovirus Expression
E. coli Expression
Molecular Biology
Products
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Products: Kinases: PAK4KD
PAK4KD (recombinant human PAK4 kinase domain)
| Catalog #: | Size: | Concentration: | Price: |
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| K0107S | 10 µg | 1 mg/ml | $490 |
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| K0107M | 100 µg | 1 mg/ml | $1,890 |
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| K0107L | 1 mg | 1 mg/ml | $6,960 |
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| K0107B | Bulk | 1 mg/ml | Inquiry |
Description:
Human PAK4 kinase (UniProt: O96013; NCBI: NM_005884) belongs to a family of p21-activated kinases. These serine/threonine kinases are regulated by the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton.
PAK4KD is a recombinant wild type human PAK4 kinase domain (amino acids 300–591 of full-length human PAK4) expressed in E. coli and purified to homogeneity with full activity.
Molecular Mass and Phosphorylation:
The calculated mass is 33,309. PAK4KD is fully phosphorylated at S474 with a measured mass of 33,389 (1P).
Stability and Storage:
Stable at -70oC for 12 months from date of receipt. Protein should be thawed on ice. Protein can be flash-frozen in liquid nitrogen and stored at -70oC.
Applications:
Enzymatic studies, inhibitor screen, and selectivity profiling
High concentration available for crystallization
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