Protein Purification
Baculovirus Expression
E. coli Expression
Molecular Biology
Products
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Products: Kinases: PAK7KD
PAK7KD (recombinant human PAK7 kinase domain, aka PAK5)
| Catalog #: | Size: | Concentration: | Price: |
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| K0108S | 10 µg | 1 mg/ml | $395 |
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| K0108M | 100 µg | 1 mg/ml | $1,500 |
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| K0108L | 1 mg | 1 mg/ml | $5,700 |
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| K0108B | Bulk | 1 mg/ml | Inquiry |
Sequence:
GPHPSRVSHEQFRAALQLVVSPGDPREYLANFIKIGEGSTGIVCIATEKHTGKQVAVKKMDLRKQQRRELLFNEVVI
MRDYHHDNVVDMYSSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIATVCLSVLRALSYLHNQGVIHRDIKSDS
ILLTSDGRIKLSDFGFCAQVSKEVPKRKSLVGTPYWMAPEVISRLPYGTEVDIWSLGIMVIEMIDGEPPYFNEPPLQA
IRRIRDSLPPRVKDLHKVSSVLRGFLDLMLVREPSQRATAQELLGHPFLKLAGPPSCIVPLMRQYRHH
- The first 4 residues GPHP are from Turbo3C cleavage site.
- Phosphorylated at S602.
Component and Formulation:
PAK7KD: 1 mg/ml in Storage Buffer of 25 mM Tris-HCl pH 8.0, 150 mM NaCl, 10% glycerol, 5 mM DTT.
Activity:
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Specific activity:
4,199 pmoles/min/µg
Analysis of enzymatic activity was performed according to the Zlyte assay protocol (Invitrogen):
- Different concentrations of PAK7KD were incubated in a buffer containing 50 mM HEPES (pH7.5), 10 mM MgCl2, 1 mM EGTA, 200 mM ATP, 0.01% Brij-35, and 2 µM substrate (SER/THR 14, Invitrogen) at room temperature for 1 hour.
- Developer solution was added to the reaction and the reaction was stopped after 1 hour of incubation at room temperature.
- Fluorescence was detected using lexc=460±40 nm and lem=528±20 nm filters.
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SDS-PAGE Analysis:
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