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Products: Eznyme Toolbox: TurboBiotinylation Kit

TurboBiotinylationTM Kit

Catalog #: Size: Concentration: Price:
B0114P 50X TurboBiotinylase (0.05 mg)
10X TurboBiotinylation Mix (1 ml)
2.5 mg/ml $300
B0114M 50X TurboBiotinylase (0.5 mg)
10X TurboBiotinylation Mix (1 ml)
2.5 mg/ml $1,800
B0114L 50X TurboBiotinylase (2.5 mg)
10X TurboBiotinylation Mix (5 ml)
2.5 mg/ml $6,000
T0114B Bulk 2.5 mg/ml Inquiry


To Order: Please email PO to order@accelagen.com or fax PO to 888-239-6490


PRODUCT DETAIL DATA SHEET PROTOCOL
Components and Formulation:
  1. 50X TurboBiotinylase at 2.5 mg/ml (80 µM) in Storage Buffer of 50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2 mM TCEP, 50% glycerol.
  2. 10X TurboBiotinylation Mix (50 mM Tris-HCl, pH 7.5, 100 mM MgCl2, 100 mM ATP, 5 mM biotin)


TurboBiotinylase Purity:

TurboBiotinylase is a stable ultra-pure recombinant E. coli BirA biotin ligase purified from E. coli and has a calculated molecular weight of 35.6 kDa. It is free of protease or nuclease contamination.


TurboBiotinylase Activity:

The efficiency of biotinylation can be measured by mass spectrometry. Biotinylation adds 226.31 Da to a protein.

Alternatively, biotinylation can be evaluated by streptavidin-agarose pull-down. A Coomassie stained gel will visually show the efficiency of biotinylation.

  1. Remove biotin and ATP from the biotinylation reaction sample by a spin desalting column.
  2. Save sample as Total (T)
  3. Incubate with streptavidin-agarose for 1 hour with agitation.
  4. Spin down the streptavidin-agarose.
  5. Save the supernatant as Unbound (U)
  6. Save the streptavidin-agarose as Bound (B)
  7. Run gel and stain with Coomassie Blue.



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