Accelagen's TurboBiotinylationTM Kit is an enhanced biotinylation kit for in vitro biotinylation of biotin-accepting proteins and recombinant proteins with AviTagTM or other biotin-accepting peptides. Combining concentrated TurboBiotinylase and stable 10X TurboBiotinylation Mix, this kit offers high efficiency in vitro biotinylation from 4-37 oC.
It does not require any special buffer for its activity and is compatible with a wide range of buffers that are most suitable for target proteins. This kit works equally well across pH 5.5-10, between 0-1 M NaCl, and in 10% glycerol.
TurboBiotinylase has a specific activity of >8,000 Units/µg.
One unit of TurboBiotinylase completely biotinylates 1 pmol of a substrate protein (23 kDa) at concentration of 1 mg/ml within 1 hour at 25 oC.
Storage and Handling:
TurboBiotinylation Kit is shipped with gel packs.
The kit retains its full activity after incubation at 25 oC for over a week.
All components of the kit can be stored at 4 oC for at least 6 months. For long term storage, store 50X TurboBiotinylase at -80 oC and 10X TurboBiotinylation Mix at 4 oC.