The Gene-to-Protein Company

Request Quote

Protein Purification

Baculovirus Expression

Mammalian Expression

E. coli Expression

Molecular Biology

Products


Products: Eznyme Toolbox: TurboLigation Kit

TurboLigationTM Kit

Catalog #: Size: Price:
L0110S 100 reactions $140
L0110M 500 reactions $535
L0110B Bulk Inquiry


To Order: Please email PO to order@accelagen.com or fax PO to 888-239-6490


PRODUCT DETAIL DATA SHEET PROTOCOL
Ligation Reaction:
  • Warm 5X TurboLigation Buffer to room temperature (22o-25oC) before setting up TurboLigation reactions.
  • Keep TurboLigase at -20oC.
  • Thaw competent cells (not included) on ice immediately before setting up TurboLigation reactions.
  • Always include a control reaction with vector only.

Set up ligation reaction in 10 µl

5X TurboLigation Buffer 2 µl
vector __ µl total vector and insert volume up to 7 µl
insert __ µl use ~1:3 molar ratio of vector to insert

adjust volume with water to 9 µl
TurboLigase 1 µl add TurboLigase last to increase efficiency

  • Mix gently and incubate at room temperature for 5 minutes. Incubation between 5 to 10 min gives the best result.
  • Chill the reaction on ice for 1 minute.
  • Use 1-3 µl of the ligation reaction for transformation of 20-50 µl competenet cells.



Protein Purification | Baculovirus Expression | Mammalian Expression | E. coli Expression | Molecular Biology | Proteins

tel: 858-678-8618     fax: 888-239-6490    toll-free: 888-365-8085     email: info@accelagen.com
Copyright © 2018 Accelagen Inc. All rights reserved.