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Products: Eznyme Toolbox: TurboNuclease

TurboNucleaseTM

recombinant Serratia marcescens extracellular endonuclease
(encoded by the same gene as Benzonase)

Catalog #: Size: Concentration: Price:
N0103M 50,000 Units 250 Units/µl $218
N0103L 250,000 Units 250 Units/µl $840
N0103B Bulk 250 Units/µl Inquiry


To Order: Please email PO to order@accelagen.com or fax PO to 888-239-6490


PRODUCT DETAIL DATA SHEET PROTOCOL
Description:
TurboNucleaseTM is an ultra-pure recombinant form of Serratia marcescens extracellular endonuclease (encoded by the same gene as Benzonase) produced in E. coli using a proprietary process. This non-specific endonuclease hydrolyzes both single- and double-stranded nucleic acids (DNA and RNA) to 5’-phosphorylated oligonucleotides of 1-4 bases in length. TurboNuclease is a highly purified homodimer of 27 kDa subunits that has exceptionally high specific activity and is free of protease activity. TurboNuclease is ideal to digest nucleic acids and to reduce viscosity during protein purification and sample preparation.

Protein Type:
Endonuclease

Source:
E. coli

Unit Definition:
One unit of TurboNuclease converts 1.0 OD260 of salmon sperm DNA into acid-soluble nucleotides in 30 minutes at 37oC in a reaction buffer of 50 mM Tris-HCl, pH 8.0 and 1 mM MgCl2. This corresponds to complete digestion of 50 µg of salmon sperm DNA into oligonucleotides. TurboNuclease has similar specific activity as Benzonase

Storage:
Store TurboNuclease at -20oC.

Endotoxin Level:
< 0.1 EU / 1,000 units of TurboNuclease as determined by the Endo-Safe LAL Assay.


US Distributors:
Fisher Scientific
Sigma-Aldrich
International Distributors:
Nacalai Tesque in Japan
MoBiTec in Europe
Expedeon in Europe

Protein Purification | Baculovirus Expression | Mammalian Expression | E. coli Expression | Molecular Biology | Proteins

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