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Protein Purification
Baculovirus Expression
Mammalian Expression
E. coli Expression
Molecular Biology
Products
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Products: Eznyme Toolbox: TurboTEV
TurboTEVTM Protease
| Catalog #: | Size: | Concentration: | Price: |
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| T0102M | 1 mg (10,000 Units) | 2 mg/ml | $489 |
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| T0102L | 10 mg (100,000 Units) | 2 mg/ml | $4,160 |
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| T0102B | Bulk | 2 mg/ml | Inquiry |
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Formulation:
2 mg/ml in 25 mM Tris-HCl, pH8.0, 50 mM NaCl, 1 mM TCEP, 50% glycerol
Protease Activity:
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A 49 kDa GST-fusion protein (C) at 1 mg/ml is incubated with TurboTEV or TEV Protease at a ratio of (1) 1:50, (2) 1:100, (3) 1:200 (w/w) in a buffer of 25 mM Tris-HCl, pH8.0, 150 mM NaCl, 14 mM b-mercaptoethanol at 4oC for 16 hours. The cleaved products are 27 kDa and 22 kDa. 'TEV' is a competitors’ TEV Protease product.
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