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Products: Eznyme Toolbox: λPPase

lambda Protein Phosphatase (λPPase)

Catalog #: Size: Concentration: Price:
P0111M 100,000 units 400,000 units/ml $430
P0111B Bulk 400,000 units/ml Inquiry


To Order: Please email PO to order@accelagen.com or fax PO to 888-239-6490


PRODUCT DETAIL DATA SHEET PROTOCOL
Description:
Lambda protein phosphatase (λPPase) is a Mn2+ dependent protein phosphatase with activity to remove phosphate groups from serine, threonine, tyrosine, and histidine residues. It is the 221 amino-acid product of the ORF221 open reading frame of bacteriophage lambda (1). Recombinant λPPase is purified from a strain of E. coli that carries the λPPase gene. λPPase has minimum 95% purity with a molecular weight of 25 kDa as determined by SDS-PAGE and Coomassie Blue staining. It has no detectable protease and nuclease activity.

Protein Type:
Peotein phosphatase

Source:
E. coli

Specific Activity:
>600,000 units/mg
1 unit of λPPase hydrolyzes 1 nmol of p-nitrophenyl phosphate per minute at 30°C in assay buffer of 50 mM HEPES pH 7.5, 100 mM NaCl. 2 mM DTT, and 1 mM MnCl2.

Storage/Handling:
Store at below -70oC. Shipped with dry ice.



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