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Protein Purification
Baculovirus Expression
Mammalian Expression
E. coli Expression
Molecular Biology
Products
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Products: Kinases: PAK3KD
PAK3KD (recombinant human PAK3 kinase domain)
| Catalog #: | Size: | Concentration: | Price: |
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| K0106S | 10 µg | 1 mg/ml | $510 |
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| K0106M | 100 µg | 1 mg/ml | $1,966 |
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| K0106L | 1 mg | 1 mg/ml | $7,240 |
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| K0106B | Bulk | 1 mg/ml | Inquiry |
Sequence:
GPHPTDEEILEKLRSIVSVGDPKKKYTRFEKIGQGASGTVYTALDIATGQEVAIKQMNLQQQPKKELIINEILVMREN
KNPNIVNYLDSYLVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALDFLHSNQVIHRDIKSDNILLG
MDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRAL
YLIATNGTPELQNPERLSAVFRDFLNRCLEMDVDRRGSAKELLQHPFLKLAKPLSSLTPLIIAAKEAIKNSSR
- The first 4 residues GPHP are from Turbo3C cleavage site.
- Phosphorylated at T421.
Component and Formulation:
PAK3KD: 1 mg/ml in Storage Buffer of 25 mM Tris-HCl pH 8.0, 150 mM NaCl, 10% glycerol, 5 mM DTT.
Activity:
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Specific activity:
18,700 pmoles/min/µg
Assay was done in a Kinase Buffer using 0.1 mg/ml PAKtide as a substrate with 20 μM ATP at 30oC for 30 min. The amount of ATP transferred was measured using Kinase-Glo reagent (Promega).
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SDS-PAGE Analysis:
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