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Products: Eznyme Toolbox: TurboLigation Kit

TurboLigationTM Kit

Catalog #: Size: Price:
L0110S 100 reactions $185
L0110M 500 reactions $705
L0110B Bulk Inquiry

To Order: Please email PO to order@accelagen.com or fax PO to 888-239-6490


PRODUCT DETAIL DATA SHEET PROTOCOL
TurboLigaseTM Unit Definition:
0.015 Weiss unit (1 cohesive end ligation unit) is defined as the amount of TurboLigaseTM required to give 50% ligation of HindIII fragments of l DNA (5´ DNA termini concentration of 0.15 µM) in a total reaction volume of 20 µl in 30 minutes at 16°C in a buffer of 50 mM Tris-HCl, pH 7.5, 10 mM MgCl2, 1 mM ATP, 10 mM dithiothreitol.

TurboLigaseTM Specific Activity and Purity:
TurboLigase has a specific activity >12,000 unit/mg.
TurboLigase is purified to homogeneity.



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