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Protein Purification
Baculovirus Expression
Mammalian Expression
E. coli Expression
Molecular Biology
Products
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Products: Eznyme Toolbox: TurboLigation Kit
TurboLigationTM Kit
| Catalog #: | Size: | Price: |
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| L0110S | 100 reactions | $177 |
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| L0110M | 500 reactions | $666 |
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| L0110B | Bulk | Inquiry |
Ligation Reaction:
- Warm 5X TurboLigation Buffer to room temperature (22o-25oC) before setting up TurboLigation reactions.
- Keep TurboLigase at -20oC.
- Thaw competent cells (not included) on ice immediately before setting up TurboLigation reactions.
- Always include a control reaction with vector only.
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| Set up ligation reaction in | 10 µl | |
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| 5X TurboLigation Buffer | 2 µl | |
| vector | __ µl | total vector and insert volume up to 7 µl |
| insert | __ µl | use ~1:3 molar ratio of vector to insert |
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adjust volume with water to | 9 µl | |
| TurboLigase | 1 µl | add TurboLigase last to increase efficiency |
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- Mix gently and incubate at room temperature for 5 minutes. Incubation between 5 to 10 min gives the best result.
- Chill the reaction on ice for 1 minute.
- Use 1-3 µl of the ligation reaction for transformation of 20-50 µl competenet cells.
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