Accelagen's TEV protease, TurboTEVTM, contains an enhanced form of a catalytic fragment of the N1a protein of Tobacco etch virus (TEV), a cysteine protease that recognizes the cleavage site of Glu-Asn-Leu-Tyr-Phe-Gln-Gly/Ser and cleaves between Gln and Gly/Ser. TurboTEV Protease is a restriction grade protease that has a robust activity at 4oC with high specificity and excellent stability. It does not require any special buffer for its activity and can be used in a buffer most suitable for the target protein. TurboTEV Protease is a 52 kDa protein with both GST and His tags so it can be removed by either Ni-chelating or Glutathione (GSH) resin.
> 10 Units/µg. 1 Unit of TurboTEV Protease cleaves >85% of 3 µg of control substrate in 1 hour at 30oC.
Store at -20oC. Shipped in gel packs.